Differential expression analysis using high-throughput RNA sequencing (RNA-seq) data is widely applied in transcriptomic studies and many software tools have been developed for this purpose. Active development of existing popular tools, together with emergence of new tools means that studies comparing the performance of differential expression analysis methods become rapidly out-of-date. In order to enable researchers to evaluate new and updated software in a timely manner, we developed DEAR-O, a user-friendly platform for performance evaluation of differential expression analysis based on RNA-seq data.


Select the data source, tools and their versions, and the number of biological replicates from drop list in both query and subject sessions. Then click the "Submit" button.

Data Source

Query Tools

Subject Tools